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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: The PRMT5-LSD1 axis confers Slug dual transcriptional activities and promotes breast cancer progression

Fig. 2

Molecular interaction between Slug, PRMT5 and LSD1. (A) Schematic diagram showing the structure of PRMT5, Slug and LSD1 and various deletion constructs used. (B) Mapping the domains of Slug required for the interaction with PRMT5. Flag-tagged full-length (FL) PRMT5 was co-expressed with Myc-tagged Slug truncations in HEK293T cells. Extracts were immunoprecipitated with Flag, bound proteins were examined by western blotting using myc. (C) Identification of the domains of Slug responsible for the interaction with LSD1. Flag-tagged LSD1-FL was co-expressed with Myc-tagged Slug truncations in HEK293T cells. After lysates were immunoprecipitated with Myc antibody, associated proteins were assessed by western blotting using Flag. (D) Mapping the domains of PRMT5 required for the interaction with Slug. HA-tagged Slug-FL was co-expressed with Myc-tagged PRMT5 truncations in HEK293T cells. Extracts were immunoprecipitated with HA, interacted proteins were examined by western blotting using Myc. (E) Identification of the domains of PRMT5 critical for the interaction with LSD1. Flag-tagged LSD1-FL was co-expressed with Myc-tagged PRMT5 truncations in HEK293T cells. Lysates were immunoprecipitated with Flag, associated proteins were examined by western blotting using Myc. (F) Mapping of the domains of LSD1 required for the interaction with PRMT5. Flag-tagged PRMT5-FL was co-expressed with Myc-tagged LSD1 truncations in HEK293T cells. Extracts were immunoprecipitated with Myc or Flag as indicated, bound proteins were examined by western blotting using Flag or Myc, respectively. (G) Identification of the domains of LSD1 important for the interaction with Slug. Flag-tagged Slug-FL was co-expressed with Myc-tagged LSD1 truncations in HEK293T cells. Extracts were immunoprecipitated with Flag, associated proteins were examined by western blotting using Myc. (H) Schematic diagram delineates the molecular interaction between Slug, PRMT5, and LSD1

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