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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Targeting TROY-mediated P85a/AKT/TBX3 signaling attenuates tumor stemness and elevates treatment response in hepatocellular carcinoma

Fig. 7

CAF-derived TGF-β1 enhances the cancer stemness via upregulating TROY. (A) Percentage of immune cells in TROYhi expression patients (B1-B20) and TROYlow expression patients (A1-A20) from TCGA database. (B) Fraction of CAF cells in TROYhi and TROYlow patients. (C) GSEA analysis of TROYhigh groups was enriched in TGFβ1 receptor binding. (D) Spheroid formation assay (left) and the spheroid numbers (right) in 8024 cells treated with PBS or TGFβ1 (5 ng/ml, 24 h). (E) Representative flow cytometry plots (left) and summaries (right) of a percentage of apoptotic cells in 8024 cells treated with PBS or TGFβ1 (5 ng/ml, 24 h). Cells were treated with Sorafenib (20 μm) for 24 h. (F) Representative flow cytometry plots (left) and summaries (right) of ALDH activity in 8024 treated with PBS or TGFβ1(24 h). (G) Western blotting of TROY, p85α, Akt, Phospho-Akt (Ser473 and Thr308) and TBX3 in 8024 and Hep3B cells treated with PBS (0 h) or TGFβ1 (5 ng/ml, 24 h, and 48 h). (H) Summary of pathological and underlying regulatory mechanisms of TROY complex in hepatocellular carcinoma

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