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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Linear DNA amplicons as a novel cancer vaccine strategy

Fig. 1

Manufacturing process of DNA amplicons. A A schematic representation showing the assembly of termini PS-modified amplicon expression vectors via a PCR device is shown. The antigen-encoding sequence, taken from a plasmid DNA, comprises a promotor, one or more ORFs and a terminator. Amplicon expression vector, encoding the selected sequence, is syntethized and then amplified through PS-modified PCR primers in order to produce a plurality of amplicon expression vectors. B A schematic representation of gene expression cassettes is shown for luciferase, conTRT and M8 constructs. C HPLC chromatogram of two representative DNA amplicons, one synthetized by means of Biolaseâ„¢ Taq (top) and one synthetized by means of MyFiâ„¢ Taq (bottom). D Electrophoresis gel of four DNA amplicons, all encoding luciferase gene (synthetized by means of Biolaseâ„¢ or MyFi.â„¢ Taq polymerase, with or w/o PS-modified primers). E A schematic representation of experimental study passages is shown

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