Fig. 4

Circ_0004087 and SND1 co-regulated the MYB transcriptional activity in PCa cells. (A) Overlapping TFs with SND1-interacting proteins predicted by String (Score ≥ 0.9) and BioGRID (Evidence ≥ 2). (B) Co-IP and western blot assays revealing the interaction between SND1 and MYB. IgG was used as a negative control. (C) The construction of a dual-luciferase reporter vector containing canonical MYB binding sites (top). The transactivation of MYB in PC-3 and DU145 cells stably transfected with MYB-overexpressing or knocking-down vectors (bottom). (D) The relative MYB activity in PCa cells as indicated measured by dual-luciferase reporter assay. Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001