Fig. 3

Translocation of JMJD2C protein from the cytoplasm into the nuclei in CRC cells in vitro. a-b Immunofluorescence detection of JMJD2Cprotein in HCT116 or LoVo cells transiently transfected with shRNA/NT vector, shRNA/JMJD2C vector, empty overexpression vector, or JMJD2C overexpression vector. c-d Western blot and quantitative assay of JMJD2C protein (nuclear and whole cell lysates) in HCT116 or LoVo cells transiently transfected with shRNA/NT vector, shRNA/JMJD2C vector, empty overexpression vector, or JMJD2C overexpression vector. *, P < 0.05; **, P < 0.01 (t test)