Fig. 4
Tmod3 truncations produced by AEP cleavage promote GBM proliferation and invasion. A Immunoblots of Tmod3 and GAPDH in U87-MG and A172 cells with or without ZsGreenl-tagged Tmod3 truncations overexpression. B, C Colony formation assay of U87-MG and A172 cells with or without ZsGreenl-tagged Tmod3 truncations overexpression. Scale bar, 100 μm. Statistical analysis of the colony formation rate (C). Triplicates in each group are the bases for the presented mean ± s.d. D, E Transwell assay of U87-MG and A172 cells with or without ZsGreenl-tagged Tmod3 truncations overexpression. Scale bar, 50 μm. Triplicates in each group are the bases for the presented mean ± s.d. F Representative coronal MRI of xenograft GBM tumors orthotopically inoculated with U87-MG cells with or without ZsGreenl-tagged Tmod3 truncations overexpression on the 16th day postoperation (n = 8 per group). G Representative H&E images from every group are shown. Scale bar, 40 μm. H The weight of the mice in every group on the day of MRI detection. The data are presented as the mean ± s.d. I The tumor volume was calculated for every group using the Coniglobus formula. The data are presented as the mean ± s.d. J Kaplan–Meier survival plots analysis of the OS for mice of the indicated groups (n = 5 per group). Comparison of survival curves was performed with Log-rank tests. NC, negative control, cells expressing ZsGreen1. OE = overexpression, cells expressing ZsGreen1-tTmod3-N or ZsGreen1-tTmod3-C. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. = no significance