Fig. 8

Nuclear tTmod3-C interacting with SND1 facilitates the growth of GBM by the RhoA/CDKs pathway. A Silver-stained gel showing immunoprecipitated ZsGreen1-tagged tTmod3-C or IgG and their bound proteins from U87-MG cells (Left panel); Immunoblot showing SND1 exist in anti-ZsGreen1-tTmod3-C immunoprecipitants (right panel). B Mass spectrometry analysis of proteins interacting with ZsGreen1-tagged tTmod3-C. The detected MS/MS peptide spectra of SND1 are shown. C Co-immunoprecipitation assays for the interaction of SND1 and ZsGreen1 or ZsGreen1-tagged tTmod3-C in HEK293 cells. D Immunoblots of SND1 in U87-MG and A172 cells treated with SND1 siRNA or siNC. E Immunoblots of the SND1/RhoA/CDKs axis in U87-MG and A172 with NC, AEP KD and AEP KD/SND1 OE. F Immunoblots of the SND1/RhoA/CDKs axis in U87-MG and A172 cells with NC, AEP KD, AEP KD/tTmod3-C res, AEP KD/tTmod3-C res/siSND1-1 and AEP KD/tTmod3-C res/siSND1-3. G CCK-8 assay of U87-MG and A172 cells with NC, AEP KD and AEP KD/SND1 OE. H CCK-8 assay of U87-MG and A172 cells with NC, AEP KD, AEP KD/tTmod3-C res, AEP KD/tTmod3-C res/siSND1-1 and AEP KD/tTmod3-C res/siSND1-3. NC = negative control, cells expressing scramble shRNA or siRNA. KD = knockdown, cells expressing AEP shRNA. ^**P < 0.01, ^***P < 0.001