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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: NCAPD3 enhances Warburg effect through c-myc and E2F1 and promotes the occurrence and progression of colorectal cancer

Fig. 4

NCAPD3 inhibited TCA cycle flux via regulating the glucose metabolism switcher E2F1. A, B Western blot analysis of the expression of indicated proteins in CRC cells with different transfections or E2F1 inhibitor HLM006474 treatment. Quantitation of proteins were presented from three independent replicates. C, D Immunoprecipitation identified the interaction between NCAPD3 and E2F1. E The reciprocal IP was performed with c-Myc or E2F1 antibody followed by WB detection of NCAPD3, c-Myc and E2F1. F, G ChIP-PCR analysis of E2F1 binding at the promoter of PDK1 and PDK3 gene loci in cells with indicated transfection. H represents HLM006474 (E2F1 inhibitor). shCTRL represents shcontrol, shNC3 represents shNCAPD3. Each experiment was performed at least triplicate and results were shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001, based on Student’s t-test or two-way ANOVA

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