Fig. 5

Leptin triggers cell cycle-related protein expression and EGFR/MAPK/c-Myc pathway in NPC. a Western blot indicated the expressions of cyclin D1, cyclin E, CDK4, p21 and p27 in leptin-overexpression and leptin-depleted TW06 cells. b Western blot analysis was performed to detect the protein levels of p-EGFR, EGFR, p-ERK1/2, ERK1/2, p-AKT, AKT, p-mTOR, mTOR and c-Myc in leptin-overexpression and leptin-depleted TW06 cells. c The IHC was performed to determine the protein expressions of leptin, p-EGFR, EGFR, p-ERK, ERK and c-Myc in the tumor tissues from shleptin-bearing xenograft model. d The EGFR mRNA levels were determined in gain-of-function of leptin in NPC cell lines. e TW06 cell lysates were subjected to EGFR immunoprecipitation with an anti-EGFR antibody or a control antibody, or subjected to leptin immunoprecipitation with an anti-leptin antibody, followed by Western blot of immunoprecipitation with an anti-leptin antibody or with an anti-EGFR antibody, respectively. f The transcriptional profiles of glycolytic molecules were examined by QPCR in leptin-overexpressed TW06 cell lines with c-Myc knockdown. n.s. no significant; *P < 0.05, **P < 0.01