Fig. 2

HSP90 inhibitor, SNX2112 induces tumor-suppressive effects in PRCC cells. A Heat map view of the high-throughput single-agent drug screening in PRCC cell lines (UOK345, UOK342). Z-transformed, area-under-the-curve (Z-AUC) values were used to assess compound potency and generate this heat-map. Heatmap ranking is based on the average compound potency in the two PRCC cell-lines tested. Highly potent HITs were defined as drugs whose Z-AUC value was < -1.5 in both cell lines (n = 110). Selected target-classes that were overrepresented among these HITs are highlighted on the top part of the plot. For any given target class, including HSP90 inhibitors, individual drugs are identified as individual black line and displayed accordingly to the average potency ranking. B Effect of SNX2112 on cell viability in PRCC. UOK345, UOK337, UOK342, UOK332 and normal RPTEC cell lines were seeded in 96 well plate in media containing 10% fetal bovine serum and incubated with a range of concentrations of SNX2112 for 72 h, and cell viability was determined using Cell-Titer Glo assay. Data are expressed as mean ± SD of triplicates and are representative of three independent experiments. *p < 0.05 is considered significant and was calculated by the two tailed Student’s t test. C SNX2112 dramatically inhibits 2D colony formation in PRCC cells. Representative images of colonies stained with crystal violet (0.5%) after 10–12 days treatment. D SNX2112 decreases 3D soft-agar colony formation in PRCC cells under anchorage-independent conditions. Representative images of colonies are shown on day 21 of treatment for each condition. Scale bars: 100 μm. E Bar graphs represent the number of soft agar colonies. Data represent mean ± SD of triplicate experiments *p < 0.05 is considered significant and was calculated by the two tailed Student’s t test or other tests