Fig. 4

SNX2112 treatment induces apoptosis and causes G2/M cell cycle arrest in PRCC cells. UOK345, UOK337, UOK342 and UOK332 cell lines were treated with SNX2112 (50 nM) or vehicle for 48 h, stained with annexin V and propidium iodide and analyzed using flow cytometry (A) Representative dot blots and (B) bar graphs expressing increased percentage of apoptotic cells after SNX2112 (50 nM) treatment. Data are mean ± SD of three independent experiments. *p < 0.05 is considered significant and was calculated by the two tailed Student’s t test. C SNX2112 treatment is associated with increased caspase 3/7 activity. UOK345, UOK337, UOK342 and UOK332 cell lines were treated with SNX2112 (50 nM) or vehicle control for 48 h and caspase 3 and 7 activity was detected by luminescent assay. Data are mean ± SD of triplicate experiments *p < 0.05 is considered significant and was calculated by the two tailed Student’s t test. D SNX2112 treatment increases levels of cleaved PARP. PRCC cell lines (UOK345, UOK342) treated with 50 nM of SNX2112 for 48 h were lysed and subjected to western blot analysis. Three independent experiments were performed, and a representative blot is shown. E SNX2112 treatment causes G2/M cell cycle arrest. UOK345 and UOK342 cells were treated with 50 nM of SNX2112 for 48 h, stained with propidium iodide, followed by flow cytometric analysis of the cell cycle distribution. F Histogram representation of the percentage of cells in different cell-cycle phases. *p < 0.05 is considered significant and was calculated by the two tailed Student’s t test