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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Ryanodine receptor 1-mediated Ca2+ signaling and mitochondrial reprogramming modulate uterine serous cancer malignant phenotypes

Fig. 3

RYR1 modulates intracellular calcium levels. A Cytosolic Ca2+ concentration (nM) monitored by Fura-2 in ARK1-shRYR1 (n = 87) or -shCtrl (n = 58) (left) and -RYR1 (n = 271) or -Control (n = 171) (right) cells. ****p < 0.0001 (Student t-test). B Mean normalized RYR1- mediated Ca2+ transient activated by 4-CMC (25 μM) in ARK1 (blue, n = 116), ARK1-shRYR1 (red, n = 70), and ARK1-RYR1 (green, n = 55) cells. C Mitochondrial Ca2+ detected by CEPIA2mt in ARK1 (blue, n = 21), ARK1- RYR1 (red, n = 16), and ARK1-shRYR1 (green, n = 13) cells activated by 4-CMC (25 μM). D Representative image of 2 ARK1 cells, indicating 4 different ROIs at which photorelease of cADPR was activated, and ER and mitochondrial Ca2+ transient was recorded. The time course of change in ER (blue) and mitochondrial (red) Ca2+ signals (F/F0) recorded in the 4 ROIs depicted in the image. E Mean normalized ER [Ca2+] and mitochondrial [Ca2+] activated by photorelease of caged cADPR at subcellular ROIs in ARK1 cells (n = 12 ROIs in 9 cells)

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