Fig. 5

RYR1 modulates mitochondrial bioenergetics properties. A Graphic illustration of ETC functions in mitochondrial membrane. B-C Representative pattern of OCR as a function over time in ARK1-shRYR1 vs shCtrl cells (B) or dantrolene- vs vehicle-treated cells (C). D-F Mitochondrial ATP and total ATP production rate (basic, pmol/min) in ARK1- shRYR1 or -shCtrl cells (D), cells treated with dantrolene or the vehicle (E), and ARK1-RYR1 or -Control cells (F). G, I, J Relative ATP levels in ARK1-shRYR1 vs shCtrl cells (G), USC RYR1 vs control cells (I), indicated cells incubated with dantrolene or vehicle (J). H, K Mitochondrial membrane potential of USC cells transfected with shRYR1 or shCtrl (H), treated with 50 μM dantrolene or vehicle (K) examined by JC-1 distribution. Green: JC-1 monomer, Red: JC-1 aggregates. L Bar charts showing relative ATP production (μmol/g) in mouse tumor tissues derived from ARK1-shRYR1 or -shCtrl cells. M-N, Relative NAD+/NADH ratios in USC cells transfected with shRYR1 or shCtrl (M) and treated with dantrolene or the vehicle (N). O-P Relative total and mitochondrial ROS in USC cells transfected with shRYR1 or shCtrl (O) and treated with dantrolene or vehicle (P) In B-P, N = 3 independent experiments. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n.s., no significance (Student t-test)