Fig. 6

RYR1 silencing suppresses TCA cycle and glycolysis. A-B Heatmaps showing relative levels (-log2) of differential metabolites in glycolysis and TCA cycle in cell lysates. ARK1-shCtrl vs ARK1-shRYR1 (A) and ARK1-vehice vs ARK1-dantrolene (B), subjected to LC-MS for metabolite measurement. C Graphic illustration of intermediate metabolites involved in glycolysis and TCA cycle. Green- labeled indicate the decreased ones in RYR1-silenced or dantrolene-treated cells than controls. D Representative patterns of ECAR from ARK1 cells transfected with shRYR1 or shCtrl. E-F Relative glutamine levels in USC cells transfected with RYR1- shRNAs or shCtrl (E) and treated with 50 μM dantrolene or vehicle (F). G Relative glutamine levels in USC cells transfected with RYR1 full-length cDNA or control vector. In D-G, data represent the mean ± SEM of 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student t-test)