Fig. 2From: Synaptotagmin 11 scaffolds MKK7–JNK signaling process to promote stem-like molecular subtype gastric cancer oncogenesisPhosphorylation of JNK induced by SYT11 overexpression. A, Proteome profile phospho-kinase array (R&D Systems) of SYT11-overexpressing SNU484 (SNU484-SYT11) cells. Stable SNU484 cells were generated by transfection of the SYT11 gene containing vector (pCMV3-HA-SYT11) or the control vector (empty vector, pCMV3-HA). B, Induction of the JNK phosphorylation in various GC cells (MKN1, PSK4 and SNU216) overexpressing SYT11. Cells were transfected with pCMV3-HA or pCMV3-HA-SYT11. SYT11 expression was analyzed by western blot. C, Increased phosphorylation of JNK and cJun by SYT11. JNK and cJun phosphorylation in SNU484-SYT11 and SNU484-EV cells was analyzed by western blot with the corresponding antibodies. The density of each band was analyzed using the ImageJ software (n = 3). D, Inhibition of SYT11-induced JNK and cJun phosphorylation in the absence of MKK7 expression (siMKK7). The level of phosphorylation of JNK and cJun was analyzed using the ImageJ software (n = 3)Back to article page