Fig. 5

JNK is involved in the SYT11-induced expression of EMT genes. A, Protein levels of the EMT genes in SNU484 cells transfected with siSYT11#8 or siScramble were analyzed by western blotting. The density of each band was analyzed using the ImageJ software (n = 3). B, EMT induction in cancer cells according to the increase of SYT11 expression level. C, Inhibition of mRNA expression of ANGPTL2, THBS4, JAM3, and Vimentin by the JNK inhibitor, SP600125, in the presence of high SYT11-expression in MKN1 cells as shown by qPCR. n = 3. ***p ≤ 0.005; **p ≤ 0.01; *p ≤ 0.05 (Student’s t-test). D and E, SNU484 and MKN1 cells were treated with 40 nM each of siRNA for 48 h. Cell viability was analyzed with the SRB assay (n = 6). ***p ≤ 0.005 (Student’s t-test). F, Invasion activity of SNU484 cells transfected with siScramble, siANGPTL2, siTHBS4, siJAM3, or siVimentin for 48 h (n = 3). G and H, Adhesion assay of SNU484 and MKN1 cells transfected with siScramble, siANGPTL2, siTHBS4, siJAM3 or siVimentin. Cells were treated with 40 nM each of siRNAs for 48 h. After trypsin treatment, the suspended cells were placed in a collagen-coated 96-well plate. After 1 hr., cells that did not adhere to the floor were washed off with PBS. After staining with SRB, the absorbance of cells bound to collagen was measured (n = 6)