Fig. 6

SYT11 is involved in GC cell proliferation and metastasis. A, In vitro growth-inhibitory effects of SYT11 knockdown in GC cells. GC cells expressing high levels of SYT11 were treated with 40 nM siSYT11 or siScramble for 48 h, and cell viability was analyzed with the SRB assay (n = 3). B, In vitro invasion assay of SNU484 cells transfected with 40 nM siSYT11 or siScramble (n = 3). C, Increase in the cell growth in NCI-N87 cells with low levels of SYT11 expression via inducing SYT11 overexpression: The expression of proteins was analyzed via western blotting. Cell viability was analyzed using SRB assay (n = 6). D, Inhibition of Spheroid formation of SNU484 cells treated with lenti-shSYT11#5 (n = 5). Scale bar, 200 μm. E, Inhibition of tumor formation by SYT11 knockdown: In vivo tumor formation was performed using SNU484 cells infected with lenti-shSYT11#5 or lenti-shControl. F, Inhibition of metastasis to the liver by shSYT11. Tail vein injection assay of SNU484 cells expressing shSYT11#5. H&E and IHC staining for Ki-67 in the liver 16 weeks after injection. Scale bar, 200 μm. ***p ≤ 0.005; **p ≤ 0.01; *p ≤ 0.05 (Student’s t-test)