Fig. 7
Antitumor effects of SYT11-ASO. A, Inhibition of SYT11 expression with an antisense oligonucleotide (ASO) of SYT11. SNU484 cells were treated with 50 nM SYT11-ASOs for 48 h. SYT11 expression was analyzed by western blot. B, Inhibition of cell viability by SYT11-ASO. SNU484 and MKN1 cells were treated with 50 nM SYT11-ASOs for 48 h. Cell viability was analyzed by the SRB assay (n = 3). C, Growth inhibition of SNU484 cells treated with various concentrations of SYT11-ASO #8 or NC-ASO. Cell growth was analyzed with the live-cell imaging system (n = 12). D, Scatter grams of FITC-Annexin V/PI staining of SNU484 cells treated with 100 nM SYT11-ASO #8 and NC-ASO. E, Caspase-3 activity of MKN1 cells treated with SYT11-ASO #8 and NC-ASO (n = 12). F, Inhibition of the invasion of SNU484 cells treated with SYT11-ASO#8 (n = 3). G, Inhibition of the expression of EMT-related proteins in SNU484 cells treated with SYT11-ASO #8 as analyzed by western blotting (n = 3). H, Reduced expression of genes downstream of SYT11 using SYT11-ASO #8 in SNU484 cells. I-K, The antitumor effects of SYT11-ASO in an in vivo xenograft model. SYT11-ASO#8 (10 mg/kg) was administered intraperitoneally in the MKN1 mouse model 5 times a week (n = 6). L. SYT11 expression in tumor tissues analyzed by western blot. ***p ≤ 0.005; **p ≤ 0.01; *p ≤ 0.05 (Student’s t-test)