Fig. 1
From: CD147 mediates epidermal malignant transformation through the RSK2/AP-1 pathway
Overexpression of CD147 in the epidermis results in spontaneous tumors formation in transgenic mice and affect the gene expression profile. A-B Schematic diagram of primers for genotyping and targeting strategies. EpiCD147-OE genotyping was carried out with Primer 1 (P1) and Primer 2 (P2). A, The 280-bp band was obtained for EpiCD147-OE genotyping (B), and DNA samples were prepared from total skin. C-F EpiCD147-OE mice grew tumors spontaneously. C Representative images of tumors from EpiCD147-OE mice (Scale bar = 1 cm). D The tumor formation rate of transgenic mice was recorded. Seventy percent (14/20) of EpiCD147-OE mice and 5% (1/20) of EpiCD147-WT mice grew one or more tumors by the endpoint of the 16-month observation. Hematoxylin & eosin staining (E) and immunohistochemistry staining of PCNA (F) were performed as described in the Materials and Methods. G The inflammatory response is elevated in EpiCD147-OE mice. Skin tissues from the same part of 6-week and 12-month EpiCD147-OE mice and EpiCD147-WT mice were collected. RNA-seq was performed as described in the Materials and Methods, and GSEA of EpiCD147-WT and EpiCD147-OE mice at 6 weeks (left panel) or 12 months of age (right panel) was performed. H Venn diagram of differentially expressed genes between 12-month EpiCD147-OE mice vs 12-month EpiCD147-WT mice and 6-week EpiCD147-OE mice vs 6-week EpiCD147-WT mice. Differentially expressed genes were analyzed using DESeq2. I CXCLs were elevated in EpiCD147-OE mice. RNA was extracted from EpiCD147-OE and EpiCD147-WT mice. RT-PCR was then performed with different primers for CXCL1, CXCL2 and CXCL10 as described in the Materials and Methods. Data from multiple experiments (n = 4) are expressed as the mean ± SD. Significant differences were evaluated using one-way Student’s t-test