Fig. 4

Knockdown of CD147 inhibits the malignant transformation of HaCaT cells. A-C Knockdown of CD147 suppresses the colony formation ability of HaCaT cells. HaCaT cells with stable knockdown of CD147 were generated by lentiviral infection. Protein was extracted from whole-cell lysates of HaCaT cells and subjected to immunoblot analysis using antibodies against CD147 as described in the Materials and Methods. GAPDH was used as a control (A). Cells were seeded into 6-well plates, and the number of colonies was counted as described in Materials and Methods (B). Representative images of HaCaT cells infected with sh-mock or sh-CD147 were presented (C). Data from multiple experiments are expressed as the mean ± SD. The significance of differences was evaluated using one-way ANOVA. D-E CD147-deficient HaCaT cells exhibit diminished migration and invasion abilities. A scratch assay was performed as described in the Materials and Methods. Representative images were taken at the indicated time points (D left panel), and bar chart graphs were shown from three independent experiments (D right panel). For the Transwell assay, the same number of cells (4 × 10⁴) was seeded into the upper layer of a chamber. The cells that migrated across the membrane were stained with crystal violet as described in the Materials and Methods (E left panel). Data represent the means (n = 4) ± SD of each group (E right panel). The significance of differences was evaluated using Student’s t-test