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Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: CD147 mediates epidermal malignant transformation through the RSK2/AP-1 pathway

Fig. 8

RSK2 inhibitor suppresses the DMBA/TPA-induced carcinogenesis through blocking MDSCs recruitment. A DMBA/TPA model was generated in EpiCD147-OE mice and treated with CX-F9 by intraperitoneal injection twice a week. Representative images of induced mouse models are shown at 16 weeks after stimulation. Scale bar = 1 cm. B-C Tumor volume (B) and numbers (C) were measured every week as described in the Materials and Methods. The tumor growth curves are shown as the mean tumor volume ± SD. The significance of differences was evaluated using one-way ANOVA. D-E The percentage of CD11b+Gr1+ MDSCs is reduced in CX-F9 treated mice. Skin lesions were subjected to flow cytometry as described in the Materials and Methods. The gating strategy (D) and bar charts of the percentage of CD11b+Gr1+ MDSCs (E) are shown. Data are presented as the mean ± SD. The significance of differences was evaluated using Student’s t-test. F CX-F9 inhibits c-fos expression in JB6 cells. Whole-cell lysates of JB6-Mock or JB6-CD147 cells stimulated with EGF and treated with/without CX-F9 were extracted and subjected to immunoblot analysis using indicated antibodies. GAPDH was used as loading control. G Schematic diagram of the role of CD147 in the malignant transformation of keratinocytes

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