Fig. 6

A Knockdown efficiency of LYL1 was evaluated in NB4 cell line and Kasumi-1 cell line by western blotting. B Knockdown efficiency of LYL1 was evaluated in NB4 cell line and Kasumi-1 cell line by qPCR. C Knockdown of LYL1 significantly inhibited the proliferation rates of NB4 cells and Kasumi-1 cells. D Flow cytometry showed that knockdown of LYL1 increased the apoptotic rates of NB4 cells. E Flow cytometry showed that knockdown of LYL1 increased the apoptotic rates of Kasumi-1 cells. F Cell viability experiment further showed that cell growth was not significantly influenced by GNE-987 (treated for 24 h) in LYL1 over-expressed AML cells, compared to LYL1 non-overexpressed AML cells