Fig. 5

SH3GLB1 enhances mitochondrial functions and TMZ resistance. A Western blot analysis showing the expression of LC3, p62, Atg5–12 complex, and OXPHOS complexes from cells with or without shSH3GLB1 that were treated with TMZ. B OCR was measured using a Seahorse Analyzer. The individual OCR parameters were measured using the indicated reagents (left). The summary graphs of mitochondrial respiration are illustrated at right and in the Supplementary Fig. S4B. C Altered dynamics of ΔΨm were detected using JC-1 dye in U87MG-R cells treated with TMZ (24 hours) and shSH3GLB1. The positive control with carbonyl cyanide m-chlorophenyl hydrazone (CCCP, 10 μM for 24 hours) indicates ΔΨm impairment. The results are illustrated in the bar graph. (D and E) The results of cell density assays are illustrated with (D) control (black) or shSH3GLB1 (gray); (E) vector (black) or SH3GLB1-overexpressing plasmid (gray) that were treated with TMZ for 72 hours. N = 3 in each group, *p < 0.05