Fig. 1

PDIA3P1 is upregulated in TMZ-resistant cell lines and promotes TMZ resistance. A Heatmap showed that PDIA3P1 was upregulated in TMZ resistance cell lines. B The expression of PDIA3P1 is higher in primary gliomas than in recurrent gliomas. C Survival analysis of GBM patients stratified by whether they received chemotherapy and expression level of PDIA3P1. D Cell viability assay of PDIA3P1-knockdown and control U118MG and U87MG cells treated with various concentrations of TMZ for 48 h. The detailed IC₅₀ were listed in the right panel. E Cell viability assay of PDIA3P1-overexpression and control LN229 and U251 cells treated with various concentrations of TMZ for 48 h. The detailed IC₅₀ were listed in the right panel. F H Bioluminescence imaging of tumor growth in xenograft nude mice with PDIA3P1 knockdown (F) or overexpression (H) and receiving or exempt from TMZ treatment in U118MG and U251 xenografts, respectively. G I The quantification of the photon counts of U118MG and U251 xenografts, respectively. The tumor sizes were monitored on day 5 and day 15