Fig. 3

Overexpression of PDIA3P1 counteracted TMZ treatment-induced DNA damage and growth inhibition. A Representative images and quantification of comet assay on ov-PDIA3P1 or Vector U251 and LN229 cells with or without TMZ treatment (400 μM, 48 h). Scale bar, 100 μm. B Representative images and quantification of γ-H2AX staining on ov-PDIA3P1 or Vector U251 and LN229 cells with or without TMZ treatment (400 μM, 48 h). Scale bar, 10 μm. C Representative images of U251 cells subjected to the EdU cell proliferation assay (left panel; scale bar, 100 μm) and quantification of EdU-positive cells (right panel) with or without TMZ treatment (400 μM, 48 h). D Apoptosis assay showing the effect of PDIA3P1 overexpression on U251 with or without TMZ treatment (400 μM, 48 h). The right panel was the quantification of apoptosis cells