Fig. 4

Elevated expression of PDIA3P1 is associated with Mesenchymal subtype. A Statistical analysis of PDIA3P1 in normal, proneural (PN) and mesenchymal (MES) tissues in the TCGA GBM dataset. B The relative expression of PDIA3P1 in NPC, PN GSCs and MES GSCs. C ROC curves of PDIA3P1 for MES-GBM subtype prediction in TCGA. D The PDIA3P1 expression is negatively correlated with PN related genes and positively correlated with MES related genes. E GSEA showed a significant positive correlation between the expression of PDIA3P1 and MES subtypes, and a negative correlation with PN subtypes. F Representative images of IF staining revealing the effect of PDIA3P1 knockdown on the expression of CD44 and SOX2 in GSC20 and GSC267, respectively. Scale bar, 10 μm. G Extreme limit dilution assays showing a decreased self-renewal ability after knockdown of PDIA3P1 in GSC20 and GSC267, respectively. H The protein expression of MES markers after PDIA3P1 knockdown in GSC20 and GSC267