Fig. 2
Drug screen highlights proteasome inhibitors as top-ranking effective drugs against HCC PDXOs. A Median cell viability of 268 drugs (treated at 1 μM) across 14 PDXO lines displayed in a box-and-whisker plot along with the variance. Dotted line indicates 50% viability. B Heatmap showing relative viability of top 20 ranked drugs for each HCC PDXO line and a non-tumorigenic liver epithelial cell control, THLE-2. Color-coded values of the heatmap represent the mean of 2 technical replicates. C Mean cell killing effects of proteasome inhibitors (bortezomib, carfilzomib, ixazomib) and common HCC clinical drugs (sorafenib, regorafenib, lenvatinib, cabozantinib, oxaliplatin, 5-FU) in 14 PDXO lines as derived from drug screen at 1 μM. Data presented as mean ± SD, n = 14. P < 0.001 comparing each proteasome inhibitor with other drugs (Kruskal-Wallis test). D IC50 values of carfilzomib, ixazomib, sorafenib, and regorafenib in HCC PDXO lines