Fig. 6

PM2.5 activates the expression of eIF4E that subsequently increases the translation of DLAT in polysomes. A PM2.5 enhanced eIF4E gene expression in BEAS-2B cells in a dose-response manner. B PM2.5 increased eIF4E gene expression in lung tissues of rats. C PM2.5 up-regulated eIF4E protein expression in BEAS-2B cells. D PM2.5 increased eIF4E protein expression in lung tissues of rats. E Up-regulation of eIF4E increased the expression of DLAT protein and knockdown of eIF4E decreased DLAT protein expression. F The expression level of eIF4E was positively correlated with that of DLAT in LUAD tumor tissues (TCGA datasets). G Correlation between eIF4E and DLAT expression levels in LUSC (TCGA datasets). H Overexpression of eIF4E increased the polysome to monosome (P/M) ratio of DLAT mRNA expression, suggesting an increase of DLAT translation initiation. I Up-regulation of eIF4E increased the abundance of DLAT mRNA in polysome but reduced DLAT expression in monosome in A549 cells. J Knockdown of eIF4E decreased the polysome to monosome ratio of DLAT mRNA expression, indicating a reduction of DLAT translation initiation. K Downregulation of eIF4E decreased the abundance of DLAT mRNA in polysome but enhanced DLAT expression in monosome in A549 cells. *P<0.05, **P<0.01