Fig. 5

Identification of circRNAs in BLCA tissues by RNA-seq analysis. A Overlap of circRNAs identified in this study and in circBank. B Genomic origin or identified circRNAs in BLCA tissues. C Length distribution of exonic circRNAs. D Heatmap of DEcircRs in three pairs of BLCA tissues. Rows: circRNAs; columns: tissues. Red, upregulation; blue, downregulation. E PCA plot of DEcircRs. Green dots, BLCA tissues; red dots, adjacent normal tissues. F Representative PCR confirmation of predicted circRNA backsplice junctions using divergent primers and host genes for circRNA, and convergent primers for cDNA and genomic DNA. G Confirmation of circRNA (cHP1BP3) backsplice junctions by Sanger sequencing. H RNase R resistance of circRNAs and GAPDH mRNA. ***, p < 0.001