Fig. 7

Pharmacological blocking of Cdc42 inhibits pulmonary metastasis of HCC in vivo. a Left: Representative bioluminescence images of pulmonary metastasis from vehicle- or ZCL278-treated mice (n = 6/group) tail vein intravenously injected with HCC cells stably overexpressing ARHGEF37. Right: The statistical results of the luminescence signals for lung metastasis. b Representative images and quantitation of metastatic nodules in the lungs. Scale bars: 50 μm. c Kaplan–Meier survival curve of mice from experiments in (a) (n = 6/group). d The control or ARHGEF37-overexpressing GFP⁺-HCC cells were treated with vehicle or ZCL278 before injection through tail veins. Lungs were assayed at 4 and 24 hours after injection (n = 6). Representative lung section images at 4 and 24 hours after injection are shown. The GFP⁺-HCC cells were detected and quantified. Scale bars: 10 μm. Each value in panel a, b and d represents the mean ± SD of six mice/group. * P < 0.05; ** P < 0.01; *** P < 0.001; ns represents not significant