Fig. 6

AGK maintains the mitochondrial morphology and function of EOC cells. A Transmission electron microscopic analysis of OVCAR3-AGK and OVCAR3-shAGK cells. Scale bar was 1 μm. B Western blotting analysis of indicated cell lines using antibodies against Tim29, hTim22, hTim9, OPA1, DRP1, MFN1, SOD2, RPL39, AGK and VDAC1. (C) Analysis of differential expressions of Tim29, Tim22, Tim9, OPA1, DRP1, MFN1 and SOD2 in Image J. Error bars represent the means ± SD of 3 independent experiments. *P < 0.05. D, E Flow cytometer analysis of the cellular ROS production of OVCAR3-AGK and OVCAR3-shAGK cells through DCFDA/H2DCDA-cellular ROS Assay. Error bars represent the means ± SD of 3 independent experiments. *P < 0.05. F mtDNA (G) Mitochondrial respiration and (H) ATP were analyzed using a mtDNA monitoring primer set, a mitochondrial respiration assay kit and an ATP assay kit respectively. N = 3 independent experiments