Fig. 7

AGK directly interacts with RPL39. A Scatter plot comparing with global gene-expression profiles of OVCAR3-AGK and OVCAR3-AGK-RNAi cells. B A functional enrichment analysis of KEGG pathways and GO upregulated in OVCAR3-AGK cells compared to OVCAR3-AGK-RNAi. C Streptavidin immunoblot analysis (left panel). A mass spectrometry analysis of Bio-ID assay (right panel). D Co-immunoprecipitation assay revealed that AGK interacted with RPL39 (total protein). E Co-immunoprecipitation assay revealed that AGK interacted with RPL39 in mitochondria (mitochondria protein). F Confocal images for AGK and RPL39 in mitochondria through Mito-Tracker. G, H The correlation between the expression of AGK and RPL39 in patients with EOC through IHC. The expression of AGK was positively correlated with the expression level of RPL39, P < 0.001. I The prognostic value of RPL39 in ovarian cancer series from the same publicly available datasets (http://kmplot.com/analysis/index.php?p= service&cancer = ovar). Kaplan–Meier curves through a univariate analysis (log-rank) of patients with EOC and a high expression of RPL39 (n = 1073) versus those with a low expression of RPL39 (n = 362) for the overall survival