Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

Fig. 4

RPRD1B occupies the promoters of c-Jun and c-Fos. A Luciferase activity assays showed that RPRD1B significantly increased both c-Jun and c-Fos transcriptional activity. B ChIP assay showing that the c-Jun and c-Fos promoters were enriched in RPRD1B-ChIPed DNA fragments but not in IgG-ChIPed controls. C An electrophoretic mobility shift assay was performed to detect the interaction between RPRD1B and c-Jun (left panel) and between RPRD1B and c-Fos (right panel) double-stranded DNA probes. D western blot analysis showing that the AP1 inhibitor SR11302 effectively decreased the expression of SREBP1, FASN, ACSS2 and FABP3 induced by RPRD1B. E Transwell migration assay showing that SR11302 inhibited the RPRD1B-induced migration of HGC27 cells. Scale bar, 200 μm. Data are presented as the means ± SD of three independent experiments. (NS, not significant; **, P < 0.01; ***, P < 0.001)

Back to article page