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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

Fig. 7

NEAT1 reduces TRIM25-mediated ubiquitination of RPRD1B. A CHX assays showed that overexpression of NEAT1 reduced the degradation of RPRD1B, in opposite, the silencing of NEAT1 accelerated the degradation of RPRD1B. GAPDH was used as a loading control. B MG132-induced accumulation of polyubiquitinated RPRD1B was decreased in NEAT1-overexpressed cells, and increased in NEAT1-silenced cells compared with control cells. GAPDH was used as a loading control. C Venn diagram showing that TRIM25 was the single overlapping protein between the set of proteins that interacted with RPRD1B and NEAT1. D Co-IP and pull-down assays confirmed the interaction between RPRD1B and TRIM25 and between NEAT1 and TRIM25. E After MG132 treatment, polyubiquitinated RPRD1B accumulation was induced by siNEAT1 but restrained by TRIM25 knockdown. F The CHX assay verified that RPRD1B degradation was enhanced by the silencing of NEAT1 but rescued by TRIM25 knockdown in GC cells. GAPDH was served as the loading control

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