Skip to main content
Fig. 8 | Journal of Experimental & Clinical Cancer Research

Fig. 8

From: Long non-coding RNA NEAT1 mediated RPRD1B stability facilitates fatty acid metabolism and lymph node metastasis via c-Jun/c-Fos/SREBP1 axis in gastric cancer

Fig. 8

Illustration of the role of the RPRD1B/c-Jun/c-Fos/SREBP1 axis in the lymph node metastasis of GC. A The expression of c-Jun, c-Fos, SREBP1, FASN, ACSS2 and FABP3 was reduced following siNEAT1 transfection and SR11302 inhibition. GAPDH was served as loading control. B Transwell migration assays showed that SR11302 and siNEAT1 inhibited the RPRD1B-induced migration of HGC27 and SGC7901 cells. Scale bar, 200 μm. C, D Analyses revealed linear regression curves and significant Pearson’s correlations of RPRD1B expression with c-Jun, c-Fos, SREBP1, FASN, ACSS2 and FABP3 expression in our cohort of GC samples. Positive correlations were confirmed in TCGA data. E IHC staining for RPRD1B, c-Jun, c-Fos, SREBP1, FASN, ACSS2 and FABP3 revealed higher levels in metastatic lymph nodes than in primary tumors from patients with GC. Oil Red O staining confirmed the presence of more lipid droplets in metastatic lymph nodes than in primary tumors from patients with GC presenting high RPRD1B expression. Scale bar, 50 μm. F Schematic diagram displaying the proposed mechanism of RPRD1B in lymph node metastasis of GC. Data are presented as the means ± SD of three independent experiments. (**, P < 0.01, ***, P < 0.001)

Back to article page