Fig. 6

GSNOR-mediated denitrosylation of VEGFD is associated with the development of lung adenocarcinoma. A, B Immunofluorescence staining for GSNOR in lungs with urethane-induced tumors and in LLC cell xenografts. C, D H&E and IHC staining for GSNOR in lungs with urethane-induced tumors. E Immunofluorescence staining for PECAM-1 in lungs with urethane-induced tumors. F Immunofluorescence staining for LYVE-1 in lungs with urethane-induced tumors. G-J Quantitative RT-PCR analyses in lungs with urethane-induced tumors. Numerical data were expressed as mean ± SD (each n = 5). Data in (A), (B), (C), (D), (E), and (F) are representative of three independent experiments. and data in (G), (H), (I), and (J) represent the mean ± SEM of triplicate samples. * p < 0.05, **p < 0.01. Student’s t test