Fig. 8

Denitrosylation of VEGFD disrupts its tumor suppressor functions. On day 24 post-inoculation, C57/BL6 mice bearing the Vector-, WT- or C277S-expressing LLC cell xenografts were used for the following assays. A-B Xenografts were harvested for imaging and weighing. C ELISA assay of VEGFD levels in the sera of C57/BL6 mice. D Western blot analyses in xenografts. E-F Immunofluorescence staining for PECAM-1 and LYVE-1 in the paraffin-embedded xenografts. (Q-J) Quantitative RT-PCR analyses in xenografts. Numerical data were expressed as mean ± SD (each n = 5). Data in (D) are representative of three independent experiments. and data in (B), (C), (G), (H), (I), and (J) represent the mean ± SEM of 5 samples. * p < 0.05, **p < 0.01. Student’s t test