Fig. 3

AKBA induces mitochondria-dependent apoptosis in U251 and U87-MG cells in a dose-dependent manner. Flow cytometry using Annexin V-FITC staining shows that AKBA increases apoptosis of U251 (a) and U87-MG (b) cells. Flow cytometry using JC-1 staining shows that AKBA reduces mitochondrial membrane potential in U251(c) and U87-MG (d) cells. Flow cytometry using caspase 3/7 live-cell staining shows that AKBA increases the activity of caspase 3/7 in U251 (e) and U87-MG (f) cells. Western blotting results show that AKBA induces expression of cleaved-caspase 3 and cleaved-PARP in U251 (g) and U87-MG (h) cells. The experiments were performed in triplicate