Fig. 1

RP11-1100 L3.8 is identified as a modulator of macrophage M2 polarization in hepatocellular carcinoma. A UMAP plot of all cells colored by each cell type in HCC patients (GSE140228). B Expression of canonical marker genes for macrophages, as shown by UMAP plot. C Volcano plots show the differentially expressed genes between tumor cells and normal cells. The most significant expressed lncRNA RP11-1100 L3.8 was highlighted by red. D Expression of RP11-1100 L3.8 in each cell type of HCC, shown by UMAP plot. E Violin plot showing the expression of RP11-1100 L3.8 in tumor-derived macrophage and monocyte-derived macrophage. F GSEA data showing the enrichment of KEGG_GLYCOLYSIS_GLUCONEOGENESIS peaks in patients with high RP11-1100 L3.8 expression compared with patients with low RP11-1100 L3.8 expression in TCGA LIHC dataset. NES, normalized enrichment score; FDR, false discovery rate. G GSEA data showing the enrichment of geneset GSE5099_CLASSICAL_M1_VS_ALTERNATIVE_M2_MACROHPAGE_DN peaks in patients with high RP11-1100 L3.8 expression compared with patients with low RP11-1100 L3.8 expression in TCGA LIHC dataset. NES, normalized enrichment score; FDR, false discovery rate. H GSEA data showing the enrichment of COATES_MACROPHAGE_M1_VS_M2_DN peaks in patients with high RP11-1100 L3.8 expression compared with patients with low RP11-1100 L3.8 expression in TCGA LIHC dataset. NES, normalized enrichment score; FDR, false discovery rate