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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: YTHDC1 is downregulated by the YY1/HDAC2 complex and controls the sensitivity of ccRCC to sunitinib by targeting the ANXA1-MAPK pathway

Fig. 3

YTHDC1 contributes to the inactivation of the ERK/MAPK signaling pathway in ccRCC cells. A and B, the Reactome enrichment analysis (A) and KEGG enrichment analysis (B) of the RNA-seq of YTHDC1 in A498 cells. P values as indicated. C and D, GSEA analysis and KEGG enrichment analysis of the TCGA-KIRC dataset. E, 786-O and A498 cells were transfected with the indicates shRNAs for 72 h. After puromycin selection, cells were harvested for western blot analysis. F, 786-O and A498 cells were transfected with the indicated plasmids for 24 h. Cells were harvested for western blot analysis. G, 786-O and A498 cells were transfected with the indicates shRNAs for 72 h. Then, cells were treated with or without LY3214996 (2 µM) and subjected to CCK-8 assay. H, 86-O and A498 cells were transfected with the indicates plasmids for 24 h. Then, cells were treated with or without LY3214996 (2 µM) and subjected to CCK-8 assay. I, 786-O and A498 cells were transfected with the indicates shRNAs for 72 h. Then, cells were treated with or without GSK1120212 (20 nM) and subjected to CCK-8 assay. J, 86-O and A498 cells were transfected with the indicates plasmids for 24 h. Then, cells were treated with or without GSK1120212 (20 nM) and subjected to CCK-8 assay

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