Fig. 2

m6A modification of circCCDC134. A and B m6A modification of circCCDC134 in the circPrimer and SRAMP prediction servers. C Motif analysis of the circCCDC134 methylation site. D ChIRP-MS showed that circCCDC134 cooperated with ALKBH5 and YTHDF2. E The GO results revealed that ALKBH5 and YTHDF2 may affect RNA stability. F and G ChIRP and H and I: RIP assays demonstrated that both ALKBH5 and YTHDF2 could interact with circCCDC134. J and K The qRT–PCR (24 tumour tissues) and IHC (5 pairs of CC tissues) results revealed that the expression of ALKBH5 was low in CC. L circCCDC134 expression was significantly negatively correlated with ALKBH5 expression in CC based on qPCR results. M and N qRT–PCR and actinomycin D assays showed that the expression and stability of circCCDC134 were decreased with ALKBH5 overexpression