Fig. 3

circCCDC134 facilitates CC proliferation and metastasis. A The qPCR results showed that Si-circCCDC134-1 and Si-circCCDC134-2 could significantly inhibit circCCDC134 expression in CC cells (ME180 and SiHA cells). B and C Colony formation and D, E and F: Transwell assays revealed the role of circCCDC134 in promoting the proliferation and metastasis of CC cells. Xenografts formed by ME180 cells bearing sh-circCCDC134 exhibited a significantly slower growth rate (G and H) and lower tumour weight (I) than scrambled controls. In the lung metastasis models (5 nude mice in the sh-NC group and 5 nude mice in the sh-circCCDC134 group), J and H: the volumes and the number of metastatic nodes were significantly decreased in the sh-circCCDC134 group