Fig. 5

circCCDC134 stimulates HIF1A transcription by recruiting p65. A The molecular simulation results supported that circCCDC134 could perfectly dock p65. The results of B: circRNA pull-down and C: RIP assays demonstrated that p65 could interact with circCCDC134, and D: no significant change was found in the p65 protein levels upon circCCDC134 knockdown. E Heatmaps and G: pie charts of p65 ChIP-seq. HIF1A was found to be the key target gene of p65. H and I p65 was enriched in the region − 1200 to − 400 bp from the transcription start site of the HIF1A promoter. p65 enrichment of the HIF1A promoter was reduced after interfering with J: p65 and K: circCCDC134 expression. L The expression of HIF1A was positively correlated with the expression of circCCDC134. M and N Immunohistochemistry showed that HIF1A was positively correlated with the expression of circCCDC134 in the xenograft growth and lung metastasis models