Fig. 6

circCCDC134 acts as a miR-503-5p sponge to regulate the expression of MYB. A and B Heatmap of miRNA-seq data of CC tissues and metastatic tissues. C, D The expression of miR-503-5p was low in 29 tumour and 9 metastatic tissues and E CC cells. F circRNA pull-down assays showed that miR-503-5p could specifically bind circCCDC134, and G: RNA FISH assays revealed that circCCDC134 and miR-503-5p colocalized in the cytoplasm. H The RIP assay illustrated that circCCDC134 and miR-503-5p enrichment was increased in the Ago2 group. I The luciferase reporter gene assay showed that luciferase activity was significantly inhibited in cells cotransfected with the miR-503-5p mimic and wild-type luciferase reporter