Fig. 1

The expression of GRK3 is upregulated in primary and metastatic GACs, which is associated with aggressive phenotypes and shorter survival. A Expression of GRK3 is shown in GAC tumor tissues compared with normal tissues from TCGA STAD and GTEX cohort (****P < 0.0001). B Expression of GRK3 is associated with poor survival of 876 GAC patients in KM plotter database (P = 1.2e-08). C Expression of GRK3 mRNA was measured by quantitative polymerase chain reaction (qPCR) and normalized to GAPDH in 23 pairs of primary GAC tissues and adjacent normal tissues. (**P < 0.001). D Immunohistochemistry (IHC) staining was performed using the GRK3 antibody in GAC tissue microarrays (TMAs) of primary tumor tissues and adjacent normal tissues. E Kaplan–Meier analysis of overall survival time according to GRK3 expression level in GAC TMAs. GAC patients with high GRK3 expression showed worse survival than patients with low expression did (P = 0.002). F&G The expression of GRK3 was analyzed with stage and lymph node metastases in our GAC cohort in TMAs. H GRK3 mRNA level was detected on normal tissues, primary tissues, and ascites cells by qPCR. **P < 0.001. I. GRK3 expression was detected using immunofluorescent staining in 22 cases of malignant ascites cells. GRK3/DAPI staining are shown from 4 representative PC samples with high GRK3 (upper panel); Dual staining of GRK3 (Alexa Fluor 555, red) and SOX9 (Alexa Fluor 488, green) are shown in another 4 representative PC samples with high GRK3 (bottom panel). Scale bar: 25µm