Fig. 3

Drug combinations significantly reduce both monolayer and multilayer culture of ACC: A Effects of the OTS167 and RGB-286638 drugs individually or in different combinations up to six days in two different ACC cell lines, SW13 (left panel) and NCI-H295R (right panel), by cell proliferation assay. B Effects of OTS167 and RGB-286638 individually and in combination on colony formation in ACC cells. Graphs represent the mean colony counts of SW13 cells treated with OTS167 (0.625 nM,1.25 nM), RGB-286638 (1.25 nM, 2.5 nM), and OTS167 and RGB-286638 combined and of NCI-H295R cells treated with OTS167 (1.25 nM, 2.5 nM), RGB-286638 (1.25 nM, 2.5 nM), and OTS167 and RGB-286638 combined. Error bars are mean ± SEM. C Effects of OTS167 and RGB-286638 individually and in combination on multicellular aggregate formation in SW13 and NCI-H295R cells