Fig. 5

Drug combination inhibits cell invasion and migration and induces EMT markers: A SW13 and NCI-H295R cells were treated with OTS167, RGB-286638, and a combination of OTS167 and RGB-286638. Migration (upper two panels) and Matrigel invasion (lower two panels) assays were performed at 24 h and 48 h for SW13 and NCI-H295R cells, respectively. Images were taken at 20X magnification. Data are representative of at least three independent experiments. B Percentages (%) of migrated and invaded cells in respect to control in each well of SW13 and NCI-H295R cells treated with OTS167, RGB-286638, and OTS167 and RGB-286638 combined. C Expression of N-cadherin and vimentin in SW13 and NCI-H295R cells treated with OTS167, RGB-286638, and OTS167 and RGB-286638 combined, analyzed by Western blot analysis. D Expression of vimentin in SW13 cells treated for 48 h and NCI-H295R cells treated for 72 h with OTS167, RGB-286638, and OTS167 and RGB-286638 combined, analyzed by immunofluorescence analysis