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Fig 2 | Journal of Experimental & Clinical Cancer Research

Fig 2

From: The neuronal protein Neuroligin 1 promotes colorectal cancer progression by modulating the APC/β-catenin pathway

Fig 2

NLGN1 promotes transendothelial migration in vitro A Tatsuky plot displaying the Z-Score distribution of NLGN1 in CRC cell lines (dataset GSE59857). Red dots indicate the samples with Z-Scores above 1.6, p <0.05. B qRTPCR of NLGN1 expression level in CRC cell lines selected from the cell bank available in our institute. Fold-change is calculated with respect to HUVEC cells, known to express high endogenous levels of the protein. C Immunoprecipitation assay was performed on HUVEC, NCI-H716, HuTu 80 and SNU-C2A cells, using an antiNLGN (L067) antibody and immunoblotting was conducted with a monoclonal antibody (4C12) able to recognize NLGN1. The band detectable at 120-kDa corresponds to NLGN1. D-H Transendothelial migration of human CRC cell line in which NLGN1 is overexpressed, HCT8 (D), HCT116 (E) and HT-29 (F), or silenced, HuTu 80 (G) and SNU-C2A (H). Cell migration was recorded in real time through the X-Celligence System for 12 hour. Histograms show the cell index in terms of percentage relative to start, at the indicated time points. A lower cell index in this representation means an increased capacity of trans-endothelial crossing. Values are expressed as mean ± SD (n=3 independent experiments performed in triplicate). Two-way ANOVA with Bonferroni test: * p < 0.05, ** p < 0.01, *** p < 0.001

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