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Fig 3 | Journal of Experimental & Clinical Cancer Research

Fig 3

From: The neuronal protein Neuroligin 1 promotes colorectal cancer progression by modulating the APC/β-catenin pathway

Fig 3

NLGN1 modulates lung metastasis outgrowth in a murine tail vein metastasis assay. 1,5x106 cells (A-D) HT-29 or HCT8 cells, pLVX and pEZNLGN1, and (E-H) HuTu 80 or SNU-C2A cells, shCTRL and shNLGN1, further infected with a CMV-Luc vector were intravenously inoculated into the tail of 6 weeks old NOD/SCID mice. After 4 weeks mice were subcutaneously inoculated with 15 mg/ml luciferine 5 minutes before the sacrifice and the lungs were surgically excised. Luciferine bioluminescence was recorded through IVIS Lumina II apparatus. Images shown in A, C, E and G are representative of 5 mice. Graphs in B and D show the bioluminescence of tumor cells as total flux while graphs in F and H show the luciferin bioluminescence as % of the area occupied by the metastatic foci respect the total organ area, measured on the explanted lungs. Values are expressed as mean ± SD, n=5 (B and D), n=12 (F), n=20 (H). Mann-Whitney test, two tailed: * p < 0.05 **, p < 0.01, *** p < 0.001

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