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Fig 4 | Journal of Experimental & Clinical Cancer Research

Fig 4

From: The neuronal protein Neuroligin 1 promotes colorectal cancer progression by modulating the APC/β-catenin pathway

Fig 4

NLGN1 induces metastasis formation by CRC cells in vivo. 1,5 × 106 cells (A-D) HCT116 pLVX and pEZNLGN1 cells and (E-H) SNU-C2A cells, further infected with a CMV-Luc vector were orthotopically inoculated into cecum of 6 weeks old NOD/SCID mice. After 8 weeks mice were subcutaneously inoculated with 15 mg/ml luciferine 5 minutes before the sacrifice and the cecum, intestin, stomach, spleen/pancreas, liver and lungs were surgically excised. Luciferine bioluminescence was recorded through IVIS Lumina II apparatus. Images of the primary tumor (cecum) in A and E andof the stomach (C) and spleen/pancreas (G) metastases are representative of 5 mice. Graphs in B, D, F and H show the bioluminescence of tumor cells as total flux. Values are expressed as mean ± SD, n=10. Mann-Whitney test, two tailed: * p < 0.05, *** p < 0.001

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